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Barcode Statistics

Read Level metrics

Pass: Reads for which all expected barcodes were found
LinkerError: Reads which were filtered because the fixed linker sequence between the barcodes could not be found
BarcodeError: Reads for which at least one barcode could not be matched against the expected sequences (whitelist)
TooShortError: Reads excluded from barcode matching because they were too short after adapter and Poly-A trimming

Barcode level metrics

Exact: The read contains an exact (no errors) match against one of the expected sequences (whitelist) for this barcode
Corrected: The barcode sequence contains at least one mismatch, but could be corrected to an unique whitelist sequence
Ambiguous: The barcode sequence has the same number of mismatches to two different whitelist sequences and is hence filtered
NoMatch: The barcode sequence cannot be matched to any whitelist sequence
Error: No barcode sequence can be extracted; typically because the linker sequence used to locate it in the read was not found

The UMI is a random sequence with no whitelist. In that case all sequences containing 'N's or pure homopolymers are Filtered, other sequences are Pass.

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